Particle electro- phoresis

Analyte

Charged colloids like latex particles, nanocapsules, cell organelles, protozoa etc.

• Sample mass : ~ 1 - 10mg solid
• Size interval : 15 nm - 3 µm

Equipment

• Device: Zetamaster S (Malvern Instruments, GB)
• Software: PCS Version 1.41
• Particularities: Titration programs for mobility curves

Evaluation methods and targets

• Electrophoretic light scattering detects colloid motion in the electric field
  => Electrophoretic mobility µ_e
  => Zetapotential ZP calculated by means of the Smoluchowski model

3D profiles of electrophoretic mobility or zetapotential can be created using titration programs for the variation of pH and electrolyte concentration.

Application examples

• Colloidal stability of dispersions and emulsions (PDF)
• Characterization of polymer colloid surface functionality (PDF)
• Indication of loading / coupling of colloidal carriers (PDF)

Contact

Dr. Bernd-R.Paulke
Research devision
Water-born Polymer Systems
phone +49 (0) 331/ 568-1159
fax +49 (0) 331/ 568-3110
email Bernd.R.Paulke@iap.fraunhofer.de

Comparative and additional methods

• Streaming potential measurements
• Titration of surface charge density